Staining-based X-ray tomography for visualizing the three dimensional micro-structure of soft-tissue specimens — ICTMS Committee Dep of Applied Maths Australian National University
  1. Schedule
  2. Advances in tomography using alternative/novel contrast modes
  3. Theme 1: Advances in tomography using alternative/novel contrast modes
  4. Staining-based X-ray tomography for visualizing the three dimensional micro-structure of soft-tissue specimens

Staining-based X-ray tomography for visualizing the three dimensional micro-structure of soft-tissue specimens (38)

Simone Ferstl 1 , Madleen Busse 1 , Mark Müller 1 , Melanie A Kimm 2 , Sebastian Allner 1 , Lorenz Hehn 1 , Franz Pfeiffer 1 2
  1. Department of Physics, Munich School of Bioengineering, Technical University of Munich, Chair of Biomedical Physics, München, Bayern, Germany
  2. Department of Diagnostic and Interventional Radiology, Klinikum rechts der Isar, Technical University of Munich, München, Bayern, Germany

To investigate the micro-morphology of biological specimens in 3D, we developed a method, whichis based on X-ray active staining and a laboratory-based nanoscopic X-ray tomography setup. We show thatthis method reproduces the tissue architecture with an at least similar level of detail as conventional lightmicroscopy. Beyond that, the volume data allows for tracking crucial structures in 3D.

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